Hi all, last night I had the same issue, I read a lot and it seems to be that there’s no solution yet.
I finally manage to get it working.
The problem, in my case, was having an external hard drive plugged in my PC, I just unplugged it, restart the PC and it worked as usual.
I came up with this after I saw someone having issues with playback and Plex changing the location of the media folder.
If you happen to have a USB, external hard drive or any kind of storage connected on any external USB port, give it a try.
Cheers