Updated iMac Plex Media Server to Sequoia 15.6.1 and now cannot connect to the server on my Roku, laptop via Chrome - and the iMac - unless I choose “Open Plex” from the server. And I can access it via phone app. Any ideas to help me get full connectivity back? Thanks!
Maybe sign out and sign in on the Roku.
Maybe clear cache and cookies on the browsers.
On the iMac do you have entries for PMS (and possibly Plex for Mac if you use it) in your macOS System Settings → Privacy & Security → Local Network? I think Sequoia has stricter settings than your previous OS for discovering devices on your LAN.
Do you have a VPN running?
See also the support article: