Do you use subtitles? I ask because I’m seeing the errors associated with subtitles in the logs before the transcoder failure, which I put a snippet below. If you are, can you try it without subtitles just to see if we are on the right track?
First a disclaimer that I don’t use subtitles, so I’m just observing what I see in the logs. Do you have multiple directory locations assigned for your TV Show library? I think what is happening is that for some reason, it is getting the audio/video (e.g. the .mkv) from F:\PlexMediaServer003\TV-Shows\(show specific folder), but then looking for the subtitle file (.srt) at D:\PlexMediaServer001\TV-Shows\(show specific folder) and isn’t finding the subtitle file.
Log Snip
Apr 12, 2020 14:55:03.129 [4572] DEBUG - We’re going to try to auto-select a subtitle.
Apr 12, 2020 14:55:03.129 [4572] DEBUG - Subtitles: Found a candidate subtitle language [eng] for a foreign film
Apr 12, 2020 14:55:03.129 [4572] DEBUG - Subtitles: Found a candidate subtitle that matches our forced / language preference. [eng] [0]. Reason [Prefer sidecar]
Apr 12, 2020 14:55:03.129 [4572] DEBUG - Audio Stream: 25533, Subtitle Stream: 28604
Apr 12, 2020 14:55:03.131 [4572] DEBUG - Found session GUID of n2m8et9sjugsrvnw87mublz6 in session start.
Apr 12, 2020 14:55:03.131 [4572] DEBUG - Cleaning directory for session n2m8et9sjugsrvnw87mublz6 ()
Apr 12, 2020 14:55:03.132 [4572] DEBUG - Starting a transcode session n2m8et9sjugsrvnw87mublz6 at offset -1.0 (state=3)
Apr 12, 2020 14:55:03.134 [4572] DEBUG - [Universal] Using local file path instead of URL: F:\PlexMediaServer003\TV-Shows\Game of Thrones\Season 1\Game of Thrones - S01-E01 - Winter Is Coming - Dual Audio - BR-RMZ 1080p x264 AAC+DTS.mkv
Apr 12, 2020 14:55:03.134 [4572] DEBUG - HTTP requesting GET http://127.0.0.1:32400/library/streams/28604?X-Plex-Token=xxxxxxxxxxxxxxxxxxxx
Apr 12, 2020 14:55:03.140 [6248] DEBUG - Request: [127.0.0.1:63546 (Loopback)] GET /library/streams/28604 (10 live) GZIP Signed-in Token (xxx@xxxx.xx)
Apr 12, 2020 14:55:03.144 [6248] ERROR - Couldn’t find the file to stream: D:\PlexMediaServer001\TV-Shows\Game of Thrones\Season 1\Game of Thrones - S01-E01 - Winter Is Coming - Dual Audio - BR-RMZ 1080p x264 AAC+DTS.en.srt
Apr 12, 2020 14:55:03.144 [5896] DEBUG - Completed: [127.0.0.1:63546] 404 GET /library/streams/28604 (10 live) GZIP 4ms 331 bytes
Apr 12, 2020 14:55:03.144 [4572] DEBUG - HTTP 404 response from GET http://127.0.0.1:32400/library/streams/28604?X-Plex-Token=xxxxxxxxxxxxxxxxxxxx
Apr 12, 2020 14:55:03.145 [4572] ERROR - Error configuring transcoder: TPU: Failed to download sub-stream to temporary file
Apr 12, 2020 14:55:03.145 [4572] DEBUG - Streaming Resource: Terminated session 0x5b8dad8:1xqj0pvsswiyiqeyemo8fbxv with reason Conversion failed. The transcoder failed to start up.
Apr 12, 2020 14:55:03.145 [6060] DEBUG - Killing job.
Apr 12, 2020 14:55:03.145 [6060] DEBUG - Job was already killed, not killing again.
Apr 12, 2020 14:55:03.145 [6060] DEBUG - Stopping transcode session n2m8et9sjugsrvnw87mublz6
Apr 12, 2020 14:55:03.145 [6264] DEBUG - Cleaning directory for session n2m8et9sjugsrvnw87mublz6 (C:\Users\Administrator\AppData\Local\Plex Media Server\Cache\Transcode\Sessions\plex-transcode-n2m8et9sjugsrvnw87mublz6-c9d5ebcf-4741-4796-b690-81c97a88e6cb)