Playback errors or transcoding issues

So I recently bought a 4TB HDD on top of the one I already own for my Rasberry PI Ubuntu run Plex Server. Previously I had 2 500 GB HDD in an Orico HDD Bay and they worked more than fine. Once I connected the new drive it wouldnt play and give me an error message off the 500 GB drives and the new 4TB. Everything on my previous 4 TB still plays fine but the new files on the new drive that I transferred from the 500 GB keeps either giving me a playback error or it exits due to transcoding issues. I already changed the location for the media in the library so I cant see why its not playing and or working properly if anyone has answers it would be greatly appreciated

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