Have you checked to confirm that your port forward is working externally? https://canyouseeme.org/
Once that is confirmed then you can continue troubleshooting the next parts. It might be worth stepping through this very helpful guide here The Basics of Remote Access Troubleshooting
That should hopefully help you confirm your setup piece by piece until you find the item that is now preventing your remote access from working. Could be something like a network, firewall or AV update/change that happened automatically.
