There’s many reasons why remote access might fail… many of those happen outside your control (e.g. changes triggered by your ISP).
Sadly, Plex is using an „optimistic“ approach with its remote access status indicator. The remote access status will be listed as „green“ while Plex is still performing the status check… this will usually take a few seconds, but in certain circumstances it could also take several minutes.
Have you verified your manual port forward is still accessible from outside your home network? You can test this using a service like canyouseeme.org. It’s also worth to compare the public IP identified by canyouseeme.org with that seen in your server‘s remote access settings and the WAN IP listed in your router.