I have opened port 32400 (TCP) for Plex in the Fritz!Box 7520 for IPv4 and IPv6. I have deactivated the relay and enabled remote access in the Plex Media Server. However, others cannot access my Plex media library. Clicking the “Try Again” button did not change anything.
If your forwarded port isn’t visible from the outside that sound like your ISP might be blocking that particular traffic.
Another scenario could be your ISP placing you in a CGNAT where your router’s WAN IP address is no longer a publicly routable IPv4 address but “hidden” within the ISP’s network.
Avast Antivirus seems to have been the problem for me. Although that has been disabled for weeks, it seems to have been a nuisance. I uninstalled the program, since then everything works as it should.